将施奈德回老家之后,四只原本亲如兄弟的神龟由于没有了敌人,于是不再想以往一样一起战斗,而是各自在喜欢的领域内钻研,以往一起行动的忍者神龟们变成了四个独来独往的“独行侠”! 这时,纽约市中发生一连窜的怪事。原来实业家马克西米林安•J•威特斯意图征服纽约市,秘密培养了一支远古怪兽组成的军队,加上恶名昭彰的忍者组织Foot Clan,各种黑暗势力又在城市的角落里蠢蠢欲动! 神龟们又处于备战状态了!他们再次联合起来,准备向黑暗势力出击!After the defeat of their old arch nemesis, The Shredder, the Turtles are needed more than ever, but Raphael, Donatello, and Michelangelo have become lost and direction less. Leonardo has gone to Central America, on the orders of the martial arts master and father figure Master Splinter, for training. Donatello and Michelangelo have started small businesses in Leonardo's absence. Meanwhile, strange things are happening in New York City. An army of ancient creatures threatens to take over the world and the Turtles must unite again to save it.